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The dataset contains integrated values of total and mesopelagic backscatter for an EK60 38 kHz echosounder, from ~25 to 1000 m depth, collected during May and June 2013 during a Norwegian trans-atlantic cruise. Data is based on integration of data cleaned with automatic filters using KORONA, and manual cleanup of remaining problems (false bottoms etc.). All backscatter deeper than 200 m were then assigned to the mesopelagic category, with backscatter shallower than 200 m reported in the total category.